一、What are lysozyme multimers?

Lysozyme multimer is a composition containing multimers. Modern biotechnology is used to modify the protein spatial structure of monomeric lysozyme, which not only maintains the enzymatic activity properties of lysozyme, but also exhibits the non-enzyme properties of lysozyme. , exponentially improving the biological properties of lysozyme.

- Source of lysozyme -

	It is the "firewall" for animal and plant health
	There are more than 13,000 types of lysozyme discovered so far

- Egg white lysozyme is the safest source for industrial production -

The lysozyme which prepared from egg white is a kind of natural protein. In 1992, the Food Additives Association of FAO/WTO recognized that the application of Lysozyme is safe for use in food. At present, the lysozyme raw materials used in medicines, foods, cosmetics and other products are all prepared from egg white.

▌ What is lysozyme Multimer

Lysozyme multimer is the abbreviation of multimeric lysozyme. Because the quality inspection standards mainly detect the activity and Multimer content, it is called lysozyme multimer for short.

▌Process flow:

Monomeric lysozyme---proportion---dissolved in a special solution---temperature and PH value---special synthetic agent---polymer lysozyme---a special substance---mixing-- -Spray granulation---(the equipment is independently developed and patented) granulation---drying---inspection---finished product.

The core technology of lysozyme and related production equipment have obtained multiple invention patents.

- 溶菌酶多聚体的分子构型 -

	X-ray diffraction revealed that the secondary structure of lysozyme (hewlett) consists of 42% helical structure, 7% folded structure, 30% turn structure and 23% coiled structure.
	Molecular weight: 28,800 Amino acids: 258 active basic protein Carry a lot of positive charge Equipotential 11.7

- Synthesis process -

Monomer lysozyme --- Dissolved in Eichhoff solution --- Control temperature and PH value --- Eichhoff crosslinking agent --- Polymer lysozyme --- spray granulation --- Low temperature drying --- Inspection --- Finished product

- Differences between Lysozyme Multimer and Monomers -

- Lysozyme Multimer mass spectrometry -

Single lysozyme standard molecular weight：14400

Identification by the Protein Research Center of Fudan University in Shanghai

- Lysozyme Multimer electrophoresis map -

Identification by the Protein Research Center of Fudan University in Shanghai

- Lysozyme Multimer electrophoresis original image -

二、Enzymatic characteristics of lysozyme oligomers

- High temperature resistance measurement -

Under the condition of 90 degrees for 2 minutes, 98% of the activity is preserved

- Safe and reliable non-specific immune factors -

三、The biological characteristics of Multimer

▌ The Five Advantages of Lysozyme Multimer

1. Enhancing non-specific immune capacity

Quickly establish an immune barrier mechanism, which can secrete highly stable IgA and SIgA antibodies and surface active factor IGF-1 activity in various mucosal layers of animals, and play a role in preventing bacterial invasion and neutralizing toxins. At the cellular level, the formation of a large number of macrophages, neutrophils, and SIgA all possess powerful phagocytic capabilities against pathogens. The defense targets of antibodies such as immunoglobulin IgM and IgG in blood, secretions, and tissue fluids are various foreign pathogens, playing an important role in maintaining animal physiological balance.

2. Antivirus

By interacting with negatively charged and acidic eggs

The white virus acts directly, forming double salts of DNA, RNA, and deproteinized proteins, inactivating the virus and preventing its replication within the body.

Indirect Immunofluorescence Detection of Lysozyme Polymers Inhibiting PRRSV Proliferation

Shanghai Veterinary Research Institute Shanghai Aikuiying Joint Study -2019

Indirect immunofluorescence detection of inhibition of PRRSV proliferation by monomeric lysozyme

Shanghai Veterinary Research Institute Shanghai Aikuiying Joint Study -2019

3、Repair of damaged tissue

4、Sterilization and antibacterial

5、Maintaining balance of gut microbiota

Promoting the growth of beneficial bacteria not only provides a good nutritional substrate for beneficial bacteria, directly promoting their growth, but also weakens the power of harmful bacteria, indirectly promoting the growth of bifidobacteria and lactobacilli in the intestine, and ultimately improving the gut microbiota. Significantly increase the height of duodenal villi, reduce the depth of jejunal crypts, increase the ratio of duodenal and jejunal villi height to crypt depth, thereby improving intestinal development and promoting nutrient absorption.

四、Report on Experiments of Polymers in Pastures

▌ The non-specific immune function of lysozyme oligomers

- The Effect of Lysozyme Multimer on the Production Performance of Growing Pigs -

The experimental results indicate that:

Compared with the control group, L100 (100mg/kg) can improve the daily weight gain and feed to meat ratio of growing pigs by replacing anti negative drugs.

Experimental at the Institute of Subtropical Agricultural Ecology, Chinese Academy of Sciences

- The effect of lysozyme oligomers on the intestinal morphology of weaned piglets -

The experimental results indicate that:

Lysozyme oligomers significantly increase the height of duodenal villi, reduce the depth of jejunal crypts, significantly increase the ratio of duodenal and jejunal villi height to crypt depth, thereby improving intestinal development and promoting nutrient absorption.

- 溶菌酶多聚体对K88攻毒仔猪肠道形态的影响 -

The experimental results indicate that:

In the attack state, the crypt depth in the duodenum and ileum of the lysozyme polymer group piglets was significantly lower than that of the control group piglets, and the ileum villous crypt ratio was significantly higher than that of the control group piglets. Lysozyme oligomers can improve the intestinal morphology of piglets infected with Escherichia coli and enhance their digestive and absorption functions.

Experimental at Chuannong Animal Nutrition Institute

- The effect of lysozyme oligomers on the gut microbiota of K88 infected piglets -

The experimental results indicate that:

Lysozyme oligomers (100mg/kg) can alleviate the impact of E. coli infection on piglet gut microbiota, improve gut microbiota balance, and enhance piglet gut health.

Experimental at Chuannong Animal Nutrition Institute

- The effect of lysozyme oligomers on the diarrhea rate of weaned piglets -

a. There is a significant difference (p<0.05) in the representation of different letters for peer data annotation, while the same or no letters indicate no significant difference (p>0.05): the result is represented as a tie value.

The experimental results indicate that:

Lysozyme polymer 120mg/kg was similar to the antibiotic group and significantly reduced the diarrhea rate of piglets compared to the control group (blank group)

Lysozyme decreased by 21.32%, 28.52%, 35.74%, and 42.79% respectively from 0 to 7 days. Reduced by 15.48%, 11.61%, 30.97%, and 34.84% respectively throughout the entire period

The 90mg/kg lysozyme group increased the average daily weight gain of weaned piglets by 35.5g compared to the control group. Diarrhea decreased by 30.97% throughout the entire period.

90mg/kg lysozyme group (calculated based on active ingredients) can replace antibiotics (20mg/kg antibiotics sulfate+50mg/kg kitasamycin, calculated based on active ingredients)

Experimental at Chuannong Animal Nutrition Institute

- Clinical Application Summary -

小猪腹股沟淋巴结观察	健仔数和初生重提高显著
猪的外观及内脏变化 添加量：1000克/吨饲料；20-40公斤生长猪；外观：皮红毛亮，体型改变，背沟明显；剖解：肝脏鲜红且富有弹性，肠道粉红且富有弹性。	成活率、断奶重提高
畸形和死胎数减少显著	母猪健康   猪就好养了

五、How to use it scientifically?

▌Application of recommended solutions in sow feed

Plan:

From the beginning of pregnancy to the end of lactation, add 1000 grams/ton of lysozyme oligomers to the feed. Repeated and continuous addition of lysozyme polymers results in better production performance.

Purpose:

1. Clinical manifestations: Eliminating constipation in sows, with loose and formed feces; Reduce bleeding points on the body surface and eliminate tear spots.

2. Production performance: The birth weight, number of healthy piglets, survival rate, birth process, weaning litter weight, and weaning rate of piglets have been significantly improved.

3. Blue ear inhibition: The addition of lysozyme oligomers throughout the process eliminates swelling of inguinal lymph nodes in piglets, effectively controls miscarriage, stillbirth, and weak offspring, and effectively controls the blue ear disease virus.

▌The effect on diarrhea and growth performance of weaned piglets

Plan:

Add 1000 grams/ton of lysozyme polymer to the feed from the start of feeding in the delivery room until the end of the 56 day old childcare period.

Purpose:

1. Clinical manifestations: shiny hair, moisturized skin, strong appetite, and formed feces;

2. Production performance: Daily weight gain, material to weight ratio, and survival rate have been significantly improved.

- Recommended Scheme for Lysozyme Polymers -

Summary：

1. Adding polymers to feed can achieve no resistance at the feed end and reduced resistance at the breeding end;

2. Adding polymers to pregnancy feed can improve the reproductive and production performance of sows;

3. Adding polymers to educational materials can reduce piglet diarrhea and promote growth;

4. Adding polymers to fertilizers can improve feed quality and reduce feed to meat ratio.